tlpA [2020-04-21 16:57:11]

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, acts also as pH sensor, responds to decreasing pH as an attractant signal

Locus: BSU_31250

Isoelectric point: 4.91

Molecular weight: 72.09 kDa

Protein length: 662 aa

Gene length: 1989 bp

Function: control of chemotaxis, sensing of acidic environments

Product: methyl-accepting chemotaxis protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Membrane-bound chemoreceptors
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,208,280 3,210,268

The protein

Paralogous protein(s)

• TlpB, McpC, McpB, McpA

Domains

• Cache domain (aa 152-228) (according to UniProt)
• HAMP domain (aa 303-355) (according to UniProt)
• Methyl-accepting transducer domain (aa 374-610) (according to UniProt)

Localization

• cell membrane PubMed, Polar (Septum) PubMed
• accumulates at strongly curved membrane areas generated during cell division PubMed

Expression and Regulation

Operons

• Genes: tlpA-mcpA
  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon

  Additional information

  • in minimal medium, McpA is present with 15,900 +/- 3,000 molecules per cell PubMed

Biological materials

Mutant

• BKE31250 (tlpA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT, downstream forward: _UP4_TAATAGGTCTAAAATACTAT
• BKK31250 (tlpA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT, downstream forward: _UP4_TAATAGGTCTAAAATACTAT

References

Reviews

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Original publications

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